Zdjęcie: Functionalization of Polycarbonate with Proteins; Open-Tubular Enzymatic Microreactors

Functionalization of Polycarbonate with Proteins; Open-Tubular Enzymatic Microreactors

This paper examines a set of techniques for the immobilization of enzymes on the surface of microchannels fabricated in polycarbonate (PC). Our experiments identify the method that uses combined physico-chemical immobilization on a layer of polyethyleneimine (PEI) as a reproducible vista for the robust immobilization of proteins. As an example, we demonstrate the fabrication, throughput and stability of an open-tubular reactor draped with alkaline phosphatase (ALP, EC 3.1.3.1) as a model enzyme. As PC is suitable for industrial applications the method could potentially be used to immobilize proteins in numbered-up implementations.

  • Autor: Ogończyk D, Jankowski P, Garstecki P
  • Rok: 2012
  • Źródło: Lab-on-a-Chip
  • Plik: pobierz

powrót »

en
Created by PONG, design Maciej Szkopański.